# Binding free energy

**URL:** <https://gromacs.bioexcel.eu/t/binding-free-energy/3744>\
**Category:** User discussions\
**Created:** [February 27, 2022, 5:11am UTC](https://gromacs.bioexcel.eu/t/binding-free-energy/3744 "2022-02-27T05:11:17Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![sadaf](https://avatars.discourse-cdn.com/v4/letter/s/77aa72/32.png) [@sadaf](https://gromacs.bioexcel.eu/u/sadaf)\
**Post date:** [February 27, 2022, 5:11am UTC](https://gromacs.bioexcel.eu/t/binding-free-energy/3744/1 "2022-02-27T05:11:17Z")

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GROMACS version:  
GROMACS modification: Yes/No  
Dear Gromacs user  
I need your kind suggestion in setting up a relative free energy calculation for mutation of an amino acid from Arginine to Histidine in the presence of cofactor.  
Could you please suggest how should I work with topology and thermodynamic cycle.  
Thanks in advance.  
Sadaf