Enforced Rotation - Fatal Error message

GROMACS version: 2020.2
GROMACS modification: No
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I have tried to use the enforced rotation approach to rotate the N-terminal Domain of the solvated protein. Then, I have received the following error message:
Fatal error:
Number of atoms in file rotref.0.trr (180116) does not match the number of
atoms in rotation group (152)!

Any suggestions? I have been used the following mdp commands:
; Enforced rotation: No or Yes
rotation = Yes
; Output frequency for angle, torque and rotation potential energy for the whole group
rot-nstrout = 10
; Output frequency for per-slab data (angles, torques and slab centers)
rot-nstsout = 10
; Number of rotation groups
rot-ngroups = 1
; Rotation group name
rot-group0 = TM_A_TM_B
; Rotation potential. Can be iso, iso-pf, pm, pm-pf, rm, rm-pf, rm2, rm2-pf, flex, flex-t, flex2, flex2-t
rot-type0 = iso-pf
; Use mass-weighting of the rotation group positions
rot-massw0 = yes
; Rotation vector, will get normalized
rot-vec0 = 0 0 1
; Rotation rate [degree/ps] and force constant [kJ/(molnm^2)]
rot-rate0 = 10.0
rot-k0 = 500.0
; Rotation rate [degree/ps] and force constant [kJ/(mol
nm^2)]
rot-rate0 = 10.0
rot-k0 = 500.0
; Slab distance for flexible axis rotation [nm]
rot-slab-dist0 = 1.5
; Minimum value of Gaussian function for the force to be evaluated (for flex* potentials)
rot-min-gauss0 = 0.001
; Value of additive constant epsilon’ [nm^2] for rm2* and flex2* potentials
rot-eps0 = 0.0001
; Fitting method to determine angle of rotation group (rmsd, norm, or potential)
rot-fit-method0 = norm
; For fit type ‘potential’, nr. of angles around the reference for which the pot. is evaluated
rot-potfit-nsteps0 = 21
; For fit type ‘potential’, distance in degrees between two consecutive angles
rot-potfit-step0 = 0.25

What did I do wrong here? Any suggestions?
Thank you in advance.

Masa

I have no experience with the option you are using, but the error is telling you that
“the reference rotref.0.trr contains all the atoms while the program is expecting to find only the rotation group”
You could try to let grompp to generate trr file for you
( see usage in http://manual.gromacs.org/documentation/current/reference-manual/special/enforced-rotation.html?highlight=usage%20 )
thus you can check what format trr should have.