# ERROR 1 \[file strong\_posre.itp, line 4414\] INQUIRY **URL:** \ **Category:** User discussions\ **Created:** [June 23, 2022, 12:42pm UTC](https://gromacs.bioexcel.eu/t/error-1-file-strong-posre-itp-line-4414-inquiry/4348 "2022-06-23T12:42:22Z")\ **Posts on this page:** 1\ **Showing post:** 17
**Author:** ![mjsubach](https://dub1.discourse-cdn.com/flex017/user_avatar/gromacs.bioexcel.eu/mjsubach/32/387_2.png) [@mjsubach](https://gromacs.bioexcel.eu/u/mjsubach)\ **Post date:** [July 12, 2022, 10:32am UTC](https://gromacs.bioexcel.eu/t/error-1-file-strong-posre-itp-line-4414-inquiry/4348/17 "2022-07-12T10:32:12Z")
Hi Justin thank you again for this past answer and again I received this same error after correcting my later other coordinate file not matching topology. For this command I did not need to copy the lipids since to my understanding the command: perl inflategro.pl system.gro 4 DPPC 14 system\_inflated.gro 5 area.dat should of generated the correct number of lipids. Below is a more detailed link to my issue (I freshly re-posted for other members to manage your time) and moreover I amended my system\_inflated.gro to match the topol.top file but received more errors, thanks if you can help. (I will try and google this exact error more since I could not find this particular error in the past forum.) > [@Fatal error: number of coordinates in coordinate file (system\_inflated.gro) does not match topology](https://gromacs.bioexcel.eu/t/fatal-error-number-of-coordinates-in-coordinate-file-system-inflated-gro-does-not-match-topology/4445): > > GROMACS version: 2022.1 GROMACS modification: No Here post your question: Membrane Protein: KALP15 in DPPC Step Three: Defining the Unit Cell & Adding Solvent 2. Pack the lipids around the protein I ran the below command: perl inflategro.pl system.gro 4 DPPC 14 system\_inflated.gro 5 area.dat this resulted in the output below: There are 20 lipids within cut-off range… 11 will be removed from the upper leaflet… 9 will be removed from the lower leaflet… I then removed these 20 lipids … --- _[View the full topic](https://gromacs.bioexcel.eu/t/error-1-file-strong-posre-itp-line-4414-inquiry/4348)._