# Error in generating RMSF graph

**URL:** <https://gromacs.bioexcel.eu/t/error-in-generating-rmsf-graph/5435>\
**Category:** User discussions\
**Tags:** mdrun, analysis-tools\
**Created:** [December 15, 2022, 1:47am UTC](https://gromacs.bioexcel.eu/t/error-in-generating-rmsf-graph/5435 "2022-12-15T01:47:30Z")\
**Posts on this page:** 1\
**Page:** 1

<div class="post-metadata">

**Author:** ![isilver](https://avatars.discourse-cdn.com/v4/letter/i/13edae/32.png) [@isilver](https://gromacs.bioexcel.eu/u/isilver)\
**Post date:** [December 15, 2022, 1:47am UTC](https://gromacs.bioexcel.eu/t/error-in-generating-rmsf-graph/5435/1 "2022-12-15T01:47:30Z")

</div>

Hi! I am trying to create RMSF graphs in which the rmsf values of two protein chains which were simulated together are overlapped instead of one being shown as a continuation after the other as shown in the picture attached. Independent indexes were made for each chain. Please advise. Thanks!

 ![RMSF_(RED)SNRPE_(BLACK)CLNS1A_NR](https://europe1.discourse-cdn.com/flex017/uploads/bioexcel1/original/2X/a/af78deb36c2b4f1746848719aef380eb216aa835.jpeg)