# Gmx do\_dssp not running

**URL:** <https://gromacs.bioexcel.eu/t/gmx-do-dssp-not-running/1704>\
**Category:** User discussions\
**Created:** [March 8, 2021, 5:26am UTC](https://gromacs.bioexcel.eu/t/gmx-do-dssp-not-running/1704 "2021-03-08T05:26:37Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![Raymond](https://avatars.discourse-cdn.com/v4/letter/r/c57346/32.png) [@Raymond](https://gromacs.bioexcel.eu/u/Raymond)\
**Post date:** [March 8, 2021, 5:26am UTC](https://gromacs.bioexcel.eu/t/gmx-do-dssp-not-running/1704/1 "2021-03-08T05:26:37Z")

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GROMACS version: 2018.1  
GROMACS modification: No

Hi, I would like to address an issue regarding the secondary structure analysis using Gromacs 2018.1. I have tried to use “gmx do\_dssp” and i chose Protein as my group for analysis.

When I chose the group “Protein”, I found that the reading frame was not running. May I know what is the issue for this? I have attached the screenshot for the reference.

 ![Screenshot (1211)](https://europe1.discourse-cdn.com/flex017/uploads/bioexcel1/original/1X/57d5b29ea846a77e6bd7d549727344561fa3faa2.png)

Thank you very much.

Sincerely,  
Raymond

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**Author:** ![garg.8](https://avatars.discourse-cdn.com/v4/letter/g/f6c823/32.png) [@garg.8](https://gromacs.bioexcel.eu/u/garg.8)\
**Post date:** [February 21, 2022, 5:59am UTC](https://gromacs.bioexcel.eu/t/gmx-do-dssp-not-running/1704/2 "2022-02-21T05:59:52Z")

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Hello Mr. Raymond,

I have found same error to find secondary structure analysis. Do you find any solution for this ?

If yes, please help me.

Thank you in advance

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<div class="post-metadata">

**Author:** ![ebriand](https://dub1.discourse-cdn.com/flex017/user_avatar/gromacs.bioexcel.eu/ebriand/32/2314_2.png) [@ebriand](https://gromacs.bioexcel.eu/u/ebriand)\
**Post date:** [February 21, 2022, 8:21am UTC](https://gromacs.bioexcel.eu/t/gmx-do-dssp-not-running/1704/3 "2022-02-21T08:21:34Z")

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Typically this happens because of a mismatch between the version of the DSSP executable and the version gromacs is expecting.

Ascertain the major version number of the DSSP executable, then run gromacs do\_dssp with argument `-ver 1`, `-ver 2` or `-ver 4` respectively. Note that support for DSSP version 4 is only available in later versions of gromacs.