# gmx\_MMPBSA energy plot vs frame

**URL:** https://gromacs.bioexcel.eu/t/gmx-mmpbsa-energy-plot-vs-frame/8106
**Category:** User discussions
**Tags:** analysis-tools
**Created:** [January 22, 2024, 3:36pm UTC](https://gromacs.bioexcel.eu/t/gmx-mmpbsa-energy-plot-vs-frame/8106 "2024-01-22T15:36:29Z")
**Posts on this page:** 1
**Page:** 1

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### Author: ![Ishrat](https://avatars.discourse-cdn.com/v4/letter/i/ec9cab/32.png) [@Ishrat](https://gromacs.bioexcel.eu/u/Ishrat)
#### Post date: [January 22, 2024, 3:36pm UTC](https://gromacs.bioexcel.eu/t/gmx-mmpbsa-energy-plot-vs-frame/8106/1 "2024-01-22T15:36:29Z")

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GROMACS version: 2023  
GROMACS modification: Yes/No  
Here post your question  
Hi everyone,  
I have done the MD simulation of protein-peptide system. I have calculated the binding energy of peptide with protein and plotted the graph between delta G and frame number. During this I found that at some frame delta G is 0 which means peptide gets dissociated from the protein. so, my question is: is this correct? can I report this plot? If yes how to justify this?  
Any suggestion on this will be highly appreciated.  
Thank you