# Gmx sham didn't work

**URL:** <https://gromacs.bioexcel.eu/t/gmx-sham-didnt-work/11845>\
**Category:** User discussions\
**Created:** [March 31, 2025, 2:12pm UTC](https://gromacs.bioexcel.eu/t/gmx-sham-didnt-work/11845 "2025-03-31T14:12:19Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![sizhe\_Li](https://avatars.discourse-cdn.com/v4/letter/s/3da27b/32.png) [@sizhe\_Li](https://gromacs.bioexcel.eu/u/sizhe_Li)\
**Post date:** [March 31, 2025, 2:12pm UTC](https://gromacs.bioexcel.eu/t/gmx-sham-didnt-work/11845/1 "2025-03-31T14:12:19Z")

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I try to run the command:  
gmx sham -f proj\_2d.xvg -ls pca12\_gibbs.xpm -lp pca12\_prob.xpm -n 100 -b 300  
But got the fault：  
Read 0 sets of 0 points, dt = 1

There are 1 bins in the 0-dimensional histogram. Beta-Emin = 0  
Ptot = 0

Back Off! I just backed up ener.xvg to ./#ener.xvg.1#

GROMACS reminds you: “Don’t pay any attention to what they write about you. Just measure it in inches.” (Andy Warhol)  
Before this command, I run “gmx covar” and “gmx anaeig” with backbone, caused by the Segmentation fault which means too many atoms in ‘Protein’.For ‘-s md.tpr -f md\_center.xtc’ are generated by ‘Protein’.