# Gmx\_wham analysis error

**URL:** <https://gromacs.bioexcel.eu/t/gmx-wham-analysis-error/6790>\
**Category:** User discussions\
**Tags:** analysis-tools\
**Created:** [July 10, 2023, 8:56am UTC](https://gromacs.bioexcel.eu/t/gmx-wham-analysis-error/6790 "2023-07-10T08:56:01Z")\
**Posts on this page:** 1\
**Page:** 1

<div class="post-metadata">

**Author:** ![Syed\_M\_A](https://avatars.discourse-cdn.com/v4/letter/s/df788c/32.png) [@Syed\_M\_A](https://gromacs.bioexcel.eu/u/Syed_M_A)\
**Post date:** [July 10, 2023, 8:56am UTC](https://gromacs.bioexcel.eu/t/gmx-wham-analysis-error/6790/1 "2023-07-10T08:56:02Z")

</div>

Dear GROMACS users, I am facing an input/output error while performing gmx wham analysis.  
I am working on miRNA and graphene systems; to calculate PMF, I did umbrella sampling and generated 80 windows, But From last few weeks I have been unable to figure out, why I am getting this error; in fact, all the files are present in the directory.  
Please let me know if you have any idea about this error message.

The error message I am getting is down here.

Working dir: /iitjfs/home/IITJMU81100/re\_us\_gt2\_2ns  
Command line:  
gmx\_mpi wham -it tpr-files.dat -if pullf-files.dat -o -hist -unit kCal

* * *

Program: gmx wham, version 2021.4  
Source file: src/gromacs/utility/futil.cpp (line 481)

File input/output error:  
umbrella0.tpr

## For more information and tips for troubleshooting, please check the GROMACS website at [Common Errors — GROMACS webpage https://www.gromacs.org documentation](http://www.gromacs.org/Documentation/Errors)

* * *

MPI\_ABORT was invoked on rank 0 in communicator MPI\_COMM\_WORLD  
with errorcode 1.