# High RMSD fluctuations

**URL:** <https://gromacs.bioexcel.eu/t/high-rmsd-fluctuations/8534>\
**Category:** User discussions\
**Created:** [March 6, 2024, 11:04am UTC](https://gromacs.bioexcel.eu/t/high-rmsd-fluctuations/8534 "2024-03-06T11:04:17Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![AF1](https://avatars.discourse-cdn.com/v4/letter/a/eada6e/32.png) [@AF1](https://gromacs.bioexcel.eu/u/AF1)\
**Post date:** [March 6, 2024, 11:04am UTC](https://gromacs.bioexcel.eu/t/high-rmsd-fluctuations/8534/1 "2024-03-06T11:04:17Z")

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![image](https://europe1.discourse-cdn.com/flex017/uploads/bioexcel1/original/2X/8/8a2da6f5132dbe6b15c5aeea131a460f7bfb9c07.png)  
Hi all  
Why is my RMSD graph coming out like this? Thank you.

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**Author:** ![FarzaneJP](https://dub1.discourse-cdn.com/flex017/user_avatar/gromacs.bioexcel.eu/farzanejp/32/1211_2.png) [@FarzaneJP](https://gromacs.bioexcel.eu/u/FarzaneJP)\
**Post date:** [March 6, 2024, 12:16pm UTC](https://gromacs.bioexcel.eu/t/high-rmsd-fluctuations/8534/2 "2024-03-06T12:16:49Z")

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Hi, Based on the results you have provided, the initial guess might be the PBC issue. I recommend visually inspecting your system using VMD to get a better understanding. If it is PBC issue you can fix it by [GROMACS 3-step workflow](http://manual.gromacs.org/current/user-guide/terminology.html#suggested-workflow).

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**Author:** ![AF1](https://avatars.discourse-cdn.com/v4/letter/a/eada6e/32.png) [@AF1](https://gromacs.bioexcel.eu/u/AF1)\
**Post date:** [March 6, 2024, 6:17pm UTC](https://gromacs.bioexcel.eu/t/high-rmsd-fluctuations/8534/3 "2024-03-06T18:17:52Z")

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Hi thank you. I will do so.