Hydrogen Mass Repartitioning-Help

GROMACS version: 2026.0
GROMACS modification: No
Hi everyone…I am trying to run a MD simulation on very large membrane protein complex that is basically embedded in a POPC bilayer. The system is solvated with water molecules and counterions as well. I am trying to run 5 replicate simulations, each of atleast 1000 ns. To speed-up my computation, I am trying to do a Hydrogen Mass Repartitioning. I am new to this and I am not sure what parameters do i tweak on my production mdp file. Looking at the resources that are available online, I could see that I have to do the following additions to my production.mdp file

mass-repartition-factor = 3

dt = 0.004 ; 4 fs

constraints = h-bonds

constraint-algorithm = lincs

lincs-order = 4

lincs-iter = 1

I am not sure if these are the best options. Can anyone who has implemented this in the past help me with this? Many thanks in advance.

Those parameters should work well in most cases. Have you tried running a simulation?