# In the umbrella sampling section their is no NVT minimization step described?

**URL:** <https://gromacs.bioexcel.eu/t/in-the-umbrella-sampling-section-their-is-no-nvt-minimization-step-described/6837>\
**Category:** User discussions\
**Tags:** mdrun\
**Created:** [July 14, 2023, 5:03am UTC](https://gromacs.bioexcel.eu/t/in-the-umbrella-sampling-section-their-is-no-nvt-minimization-step-described/6837 "2023-07-14T05:03:34Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Manish](https://avatars.discourse-cdn.com/v4/letter/m/a6a055/32.png) [@Manish](https://gromacs.bioexcel.eu/u/Manish)\
**Post date:** [July 14, 2023, 5:03am UTC](https://gromacs.bioexcel.eu/t/in-the-umbrella-sampling-section-their-is-no-nvt-minimization-step-described/6837/1 "2023-07-14T05:03:34Z")

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GROMACS version:  
GROMACS modification: Yes/No  
Here post your question  
In the umbrella sampling section their is no NVT minimization step described? Why is it so. And is it required to do it or not.

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**Author:** ![Karis](https://avatars.discourse-cdn.com/v4/letter/k/ed8c4c/32.png) [@Karis](https://gromacs.bioexcel.eu/u/Karis)\
**Post date:** [July 14, 2023, 5:42am UTC](https://gromacs.bioexcel.eu/t/in-the-umbrella-sampling-section-their-is-no-nvt-minimization-step-described/6837/2 "2023-07-14T05:42:04Z")

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The NVT equilibration had already been done to prepare the .pdb file provided in the tutorial ([How to apply pulling force in one helix of a single chain globular protein to perform SMD? - #10 by jalemkul](https://gromacs.bioexcel.eu/t/how-to-apply-pulling-force-in-one-helix-of-a-single-chain-globular-protein-to-perform-smd/5803/10))