# Ligand addition to a membrane protein - lipid bilayer system and Gromacs Simulation?

**URL:** <https://gromacs.bioexcel.eu/t/ligand-addition-to-a-membrane-protein-lipid-bilayer-system-and-gromacs-simulation/4768>\
**Category:** User discussions\
**Created:** [September 10, 2022, 1:34pm UTC](https://gromacs.bioexcel.eu/t/ligand-addition-to-a-membrane-protein-lipid-bilayer-system-and-gromacs-simulation/4768 "2022-09-10T13:34:34Z")\
**Posts on this page:** 1\
**Page:** 1

<div class="post-metadata">

**Author:** ![sd206](https://avatars.discourse-cdn.com/v4/letter/s/ecd19e/32.png) [@sd206](https://gromacs.bioexcel.eu/u/sd206)\
**Post date:** [September 10, 2022, 1:34pm UTC](https://gromacs.bioexcel.eu/t/ligand-addition-to-a-membrane-protein-lipid-bilayer-system-and-gromacs-simulation/4768/1 "2022-09-10T13:34:34Z")

</div>

GROMACS version:2018

I used CHARMM GUI to incorporate the protein into the membrane lipid bilayer after sugar addition, now I want to add a ligand to the system using GROMACS, is there any step-by-step procedure for the ligand addition followed by GROMACS Simulation.

Thank you very much in advance.