# PBC changing drastically vertical in replica of a membrane protein with plumed

**URL:** <https://gromacs.bioexcel.eu/t/pbc-changing-drastically-vertical-in-replica-of-a-membrane-protein-with-plumed/12890>\
**Category:** User discussions\
**Created:** [November 9, 2025, 12:16am UTC](https://gromacs.bioexcel.eu/t/pbc-changing-drastically-vertical-in-replica-of-a-membrane-protein-with-plumed/12890 "2025-11-09T00:16:05Z")\
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**Author:** ![bjwiley233](https://avatars.discourse-cdn.com/v4/letter/b/f17d59/32.png) [@bjwiley233](https://gromacs.bioexcel.eu/u/bjwiley233)\
**Post date:** [November 10, 2025, 4:50am UTC](https://gromacs.bioexcel.eu/t/pbc-changing-drastically-vertical-in-replica-of-a-membrane-protein-with-plumed/12890/2 "2025-11-10T04:50:55Z")

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Answer could be as simple as since I made a new box with wider x and y dimensions without adding more lipids being the reason. Increasing by 1 nm on each of the x and y axis required adding 50 more DPPC molecules per leaf.

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