# pH dependent simulation

**URL:** <https://gromacs.bioexcel.eu/t/ph-dependent-simulation/13766>\
**Category:** User discussions\
**Created:** [October 11, 2026, 4:38pm UTC](https://gromacs.bioexcel.eu/t/ph-dependent-simulation/13766 "2026-10-11T16:38:04Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Trideep](https://avatars.discourse-cdn.com/v4/letter/t/8c91f0/32.png) [@Trideep](https://gromacs.bioexcel.eu/u/Trideep)\
**Post date:** [October 11, 2026, 4:38pm UTC](https://gromacs.bioexcel.eu/t/ph-dependent-simulation/13766/1 "2026-10-11T16:38:04Z")

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GROMACS version:2020.6  
GROMACS modification: Yes/No  
How to do pH dependent simulation using gromacs? I want to simulate a DNA and a ligand under two different pH environments?

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**Author:** ![daviddesancho](https://avatars.discourse-cdn.com/v4/letter/d/cdc98d/32.png) [@daviddesancho](https://gromacs.bioexcel.eu/u/daviddesancho)\
**Post date:** [October 14, 2026, 9:05am UTC](https://gromacs.bioexcel.eu/t/ph-dependent-simulation/13766/2 "2026-10-14T09:05:48Z")

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Hi Trideep

There is abundant information online on how to do pH-dependent simulations. You can start watching this [YouTube lecture](https://youtu.be/YQ8eRyScuyk?si=8F9UtSqRan5ksswn) by Noora Aho and follow the extensive literature on the topic.

All the best,

d.