# Protein at biphase interface

**URL:** <https://gromacs.bioexcel.eu/t/protein-at-biphase-interface/7950>\
**Category:** User discussions\
**Created:** [January 1, 2024, 4:36am UTC](https://gromacs.bioexcel.eu/t/protein-at-biphase-interface/7950 "2024-01-01T04:36:42Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![SP06](https://avatars.discourse-cdn.com/v4/letter/s/ecb155/32.png) [@SP06](https://gromacs.bioexcel.eu/u/SP06)\
**Post date:** [January 1, 2024, 4:36am UTC](https://gromacs.bioexcel.eu/t/protein-at-biphase-interface/7950/1 "2024-01-01T04:36:42Z")

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GROMACS version:2021.4  
GROMACS modification: Yes/No  
Here post your question : I want to simulate my protein at water-cyclohexane interface. Can I use gmx insert-molecules to insert cyclohexane molecules in a box with protein centered in it, and then add water, instead of adding a pre-equilibrated box of cyclohexane followed by adding water (as given in gromacs tutorial page for biphasic systems). Can anyone please help with this?