# Protein-ligand simulation with two identical ligands in GROMACS

**URL:** <https://gromacs.bioexcel.eu/t/protein-ligand-simulation-with-two-identical-ligands-in-gromacs/10166>\
**Category:** User discussions\
**Created:** [September 16, 2024, 7:33am UTC](https://gromacs.bioexcel.eu/t/protein-ligand-simulation-with-two-identical-ligands-in-gromacs/10166 "2024-09-16T07:33:04Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Mohammadhossein](https://avatars.discourse-cdn.com/v4/letter/m/eada6e/32.png) [@Mohammadhossein](https://gromacs.bioexcel.eu/u/Mohammadhossein)\
**Post date:** [September 16, 2024, 7:33am UTC](https://gromacs.bioexcel.eu/t/protein-ligand-simulation-with-two-identical-ligands-in-gromacs/10166/1 "2024-09-16T07:33:04Z")

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GROMACS version:  
GROMACS modification: Yes/No  
Here post your question  
Hi  
I want to perform a protein-ligand simulation.  
how can i conduct the simulation with two identical ligands in such a way that they not overlap spatially?  
How can i make complex proteine-ligand file  
Thank you.