# Relative binding free energy calculation tutorial

**URL:** <https://gromacs.bioexcel.eu/t/relative-binding-free-energy-calculation-tutorial/1619>\
**Category:** User discussions\
**Created:** [February 19, 2021, 7:23am UTC](https://gromacs.bioexcel.eu/t/relative-binding-free-energy-calculation-tutorial/1619 "2021-02-19T07:23:56Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![kexul](https://avatars.discourse-cdn.com/v4/letter/k/73ab20/32.png) [@kexul](https://gromacs.bioexcel.eu/u/kexul)\
**Post date:** [February 19, 2021, 7:23am UTC](https://gromacs.bioexcel.eu/t/relative-binding-free-energy-calculation-tutorial/1619/1 "2021-02-19T07:23:56Z")

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Hi everyone,

could someone suggest a good/recent tutorial to calculate relative binding free energy by GROMACS? Especially with some example of setting lambada value.

Thanks

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**Author:** ![Maggie](https://avatars.discourse-cdn.com/v4/letter/m/9d8465/32.png) [@Maggie](https://gromacs.bioexcel.eu/u/Maggie)\
**Post date:** [February 19, 2021, 8:56am UTC](https://gromacs.bioexcel.eu/t/relative-binding-free-energy-calculation-tutorial/1619/2 "2021-02-19T08:56:23Z")

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I used this one, maybe it helps: [Free Energy Calculations](http://www.mdtutorials.com/gmx/free_energy/index.html). Or just google ‘Gromacs Free Energy Tutorial’.

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**Author:** ![kexul](https://avatars.discourse-cdn.com/v4/letter/k/73ab20/32.png) [@kexul](https://gromacs.bioexcel.eu/u/kexul)\
**Post date:** [February 19, 2021, 9:09am UTC](https://gromacs.bioexcel.eu/t/relative-binding-free-energy-calculation-tutorial/1619/3 "2021-02-19T09:09:21Z")

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Thanks for your reply, but this one is not what I needed. The tutorial only talked about absolute free energy calculation, and the scenario was quite simplified.