# Results of umbrella sampling

**URL:** <https://gromacs.bioexcel.eu/t/results-of-umbrella-sampling/2040>\
**Category:** User discussions\
**Tags:** analysis-tools\
**Created:** [April 28, 2021, 10:35am UTC](https://gromacs.bioexcel.eu/t/results-of-umbrella-sampling/2040 "2021-04-28T10:35:24Z")\
**Posts on this page:** 4\
**Page:** 1

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**Author:** ![newbie](https://dub1.discourse-cdn.com/flex017/user_avatar/gromacs.bioexcel.eu/newbie/32/283_2.png) [@newbie](https://gromacs.bioexcel.eu/u/newbie)\
**Post date:** [April 28, 2021, 10:35am UTC](https://gromacs.bioexcel.eu/t/results-of-umbrella-sampling/2040/1 "2021-04-28T10:35:24Z")

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Hello.

The followings are the results of umbrella sampling.

 ![AAA](https://europe1.discourse-cdn.com/flex017/uploads/bioexcel1/original/1X/4d2f64f7fb0613a9a44dfafbe5b473416d5a7e74.png) ![bb](https://europe1.discourse-cdn.com/flex017/uploads/bioexcel1/original/1X/9fe4305ee3720aa0d38c1f0550fac27ac909e3c0.png)

How can I achieve the PMF, which is similar to the one in the tutorial?  
I mean in my plots, x-axes are not the same, while in tutorial both have the same x-axes.

Thank you.

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**Author:** ![alevilla](https://dub1.discourse-cdn.com/flex017/user_avatar/gromacs.bioexcel.eu/alevilla/32/439_2.png) [@alevilla](https://gromacs.bioexcel.eu/u/alevilla)\
**Post date:** [April 29, 2021, 1:12pm UTC](https://gromacs.bioexcel.eu/t/results-of-umbrella-sampling/2040/2 "2021-04-29T13:12:45Z")

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Hi,  
Yes, your first plot does not correspond to the second one. Maybe the legend of the first plot is wrong. It is difficult to answer to the post. There are many GROMACS tutorials around.  
Best regards  
Alessandra

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<div class="post-metadata">

**Author:** ![newbie](https://dub1.discourse-cdn.com/flex017/user_avatar/gromacs.bioexcel.eu/newbie/32/283_2.png) [@newbie](https://gromacs.bioexcel.eu/u/newbie)\
**Post date:** [May 2, 2021, 8:32am UTC](https://gromacs.bioexcel.eu/t/results-of-umbrella-sampling/2040/3 "2021-05-02T08:32:06Z")

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Thank you for your response.  
I followed the umbrella sampling tutorial, which is written by Dr. Lemkul. Nonetheless, I made a few changes to the pull code. I don’t know whether it is the case or not.

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**Author:** ![alevilla](https://dub1.discourse-cdn.com/flex017/user_avatar/gromacs.bioexcel.eu/alevilla/32/439_2.png) [@alevilla](https://gromacs.bioexcel.eu/u/alevilla)\
**Post date:** [May 4, 2021, 9:26am UTC](https://gromacs.bioexcel.eu/t/results-of-umbrella-sampling/2040/4 "2021-05-04T09:26:36Z")

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Hi,  
I am not familiar with this tutorial. But when you post process the data using gmx wham you get the histogram you reported but a file profile.xvg that contains the pmf curve.  
Alessandra