# RMSF graph

**URL:** <https://gromacs.bioexcel.eu/t/rmsf-graph/11577>\
**Category:** User discussions\
**Created:** [February 18, 2025, 4:41am UTC](https://gromacs.bioexcel.eu/t/rmsf-graph/11577 "2025-02-18T04:41:11Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![LIKITHA](https://avatars.discourse-cdn.com/v4/letter/l/2acd7d/32.png) [@LIKITHA](https://gromacs.bioexcel.eu/u/LIKITHA)\
**Post date:** [February 18, 2025, 4:41am UTC](https://gromacs.bioexcel.eu/t/rmsf-graph/11577/1 "2025-02-18T04:41:12Z")

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GROMACS version: 2022.2

I am generating the rmsf plots and they look different from what I expected. What could be the cause for the abrupt graph. I am adding the screenshot of the same. Please help me with this.

 ![image](https://europe1.discourse-cdn.com/flex017/uploads/bioexcel1/original/2X/c/ce27fb62a14ea491b78fde847201ccf158c25f8a.png)

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**Author:** ![nilanjana88](https://avatars.discourse-cdn.com/v4/letter/n/59ef9b/32.png) [@nilanjana88](https://gromacs.bioexcel.eu/u/nilanjana88)\
**Post date:** [February 18, 2025, 2:54pm UTC](https://gromacs.bioexcel.eu/t/rmsf-graph/11577/2 "2025-02-18T14:54:34Z")

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It seems like there are multiple chains with overlapping residues.  
If you want to check rmsf for a particular chain, create the index file for that and then run the command again with “-n index.ndx” flag.