# Running simulation of protein with Iron sulphur cluster

**URL:** <https://gromacs.bioexcel.eu/t/running-simulation-of-protein-with-iron-sulphur-cluster/4482>\
**Category:** User discussions\
**Created:** [July 21, 2022, 6:04am UTC](https://gromacs.bioexcel.eu/t/running-simulation-of-protein-with-iron-sulphur-cluster/4482 "2022-07-21T06:04:44Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Bidisha](https://avatars.discourse-cdn.com/v4/letter/b/df705f/32.png) [@Bidisha](https://gromacs.bioexcel.eu/u/Bidisha)\
**Post date:** [July 21, 2022, 6:04am UTC](https://gromacs.bioexcel.eu/t/running-simulation-of-protein-with-iron-sulphur-cluster/4482/1 "2022-07-21T06:04:44Z")

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GROMACS version:2018  
GROMACS modification: Yes/No  
Here post your question

Hello everyone,  
I am new to GROMACS and I am trying to run a protein simulation with Iron-sulfur cluster as a ligand using Amber ff99sb forcefield. Can anyone help me regarding the generation of topology files of Iron Sulfur cluster? and how to define it to the force field? Thank you very much for your help.