# Simulated Annealing for a protein-ligand

**URL:** <https://gromacs.bioexcel.eu/t/simulated-annealing-for-a-protein-ligand/8153>\
**Category:** User discussions\
**Created:** [January 26, 2024, 2:05pm UTC](https://gromacs.bioexcel.eu/t/simulated-annealing-for-a-protein-ligand/8153 "2024-01-26T14:05:22Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Rochik](https://avatars.discourse-cdn.com/v4/letter/r/ecc23a/32.png) [@Rochik](https://gromacs.bioexcel.eu/u/Rochik)\
**Post date:** [January 26, 2024, 2:05pm UTC](https://gromacs.bioexcel.eu/t/simulated-annealing-for-a-protein-ligand/8153/1 "2024-01-26T14:05:22Z")

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Dear all,  
I performed a 300-ns MD simulation for a system of protein-ligand, now I want to increase the temperature (from 300 to 330) using “single simulated annealing”, and continue my MD for a 100 ns.  
The problem is that as I want to continue my MD, I should update my .tpr file to a new one. But, I don’t know how to add the simulated annealing parameters into the .tpr file. Basically, we need to add these parameters in the md.mdp file, but I don’t want to start an MD. I want to continue my previous MD.  
Does anybody know how to deal with this issue?  
Thank you