# While trying to apply force field, i got this error

**URL:** <https://gromacs.bioexcel.eu/t/while-trying-to-apply-force-field-i-got-this-error/10875>\
**Category:** User discussions\
**Created:** [December 21, 2024, 8:28am UTC](https://gromacs.bioexcel.eu/t/while-trying-to-apply-force-field-i-got-this-error/10875 "2024-12-21T08:28:31Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![Deep](https://avatars.discourse-cdn.com/v4/letter/d/ce7236/32.png) [@Deep](https://gromacs.bioexcel.eu/u/Deep)\
**Post date:** [December 21, 2024, 8:28am UTC](https://gromacs.bioexcel.eu/t/while-trying-to-apply-force-field-i-got-this-error/10875/1 "2024-12-21T08:28:31Z")

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Processing chain 1 ‘A’ (1364 atoms, 163 residues)  
Analysing hydrogen-bonding network for automated assignment of histidine  
protonation. 273 donors and 248 acceptors were found.  
There are 347 hydrogen bonds  
Will use HISD for residue 31

Identified residue MET1 as a starting terminus.

Identified residue ASN163 as a ending terminus.  
8 out of 8 lines of specbond.dat converted successfully  
Special Atom Distance matrix:  
MET1 MET6 HIS31 CYS54 CYS97 MET106  
SD10 SD56 NE2276 SG446 SG808 SD883  
MET6 SD56 0.631  
HIS31 NE2276 2.196 1.931  
CYS54 SG446 2.781 2.718 1.052  
CYS97 SG808 0.820 0.539 2.117 2.897  
MET106 SD883 1.900 1.314 1.848 2.841 1.635  
MET120 SD982 2.525 2.014 3.286 4.293 2.112 1.520  
Start terminus MET-1: MET1  
End terminus ASN-163: COO-

* * *

Program: gmx pdb2gmx, version 2021.4-Ubuntu-2021.4-2  
Source file: src/gromacs/gmxpreprocess/pdb2top.cpp (line 1104)

Fatal error:  
atom C1 not found in buiding block 1MET while combining tdb and rtp

For more information and tips for troubleshooting, please check the GROMACS  
website at [Common Errors — GROMACS webpage https://www.gromacs.org documentation](http://www.gromacs.org/Documentation/Errors)

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**Author:** ![jalemkul](https://dub1.discourse-cdn.com/flex017/user_avatar/gromacs.bioexcel.eu/jalemkul/32/18_2.png) [@jalemkul](https://gromacs.bioexcel.eu/u/jalemkul)\
**Post date:** [December 21, 2024, 12:03pm UTC](https://gromacs.bioexcel.eu/t/while-trying-to-apply-force-field-i-got-this-error/10875/2 "2024-12-21T12:03:19Z")

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Posted yesterday (please Google or search the forum first):

> [@Fatal error atom C2 not found in buiding block 166MET while combining tdb and rtp](https://gromacs.bioexcel.eu/t/fatal-error-atom-c2-not-found-in-buiding-block-166met-while-combining-tdb-and-rtp/10860/2):
>
> You need to interactively select terminal patching with the -ter option, otherwise an incorrect terminus is guessed (quirk of the CHARMM force field and a niche patch that is sometimes used).

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**Author:** ![Deep](https://avatars.discourse-cdn.com/v4/letter/d/ce7236/32.png) [@Deep](https://gromacs.bioexcel.eu/u/Deep)\
**Post date:** [December 23, 2024, 9:06am UTC](https://gromacs.bioexcel.eu/t/while-trying-to-apply-force-field-i-got-this-error/10875/3 "2024-12-23T09:06:41Z")

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Thank You sir
