PBC changing drastically vertical in replicas of membrane protein with plumed

[bjwiley233]

Sorry I posted in wrong spot and post here now. It also happens without plumed flag. Initially start at 10.15726 10.15726 11.86791 after equilibration and by 27 nanoseconds goes to 8.82787 8.82787 15.53842.

PBC changing drastically vertical in replica of a membrane protein with plumed

Answer could be as simple as since I made a new box with wider x and y dimensions without adding more lipids being the reason. Increasing by 1 nm on each of the x and y axis required adding 50 more DPPC molecules per leaf.