I try to run the command:
gmx sham -f proj_2d.xvg -ls pca12_gibbs.xpm -lp pca12_prob.xpm -n 100 -b 300
But got the fault：
Read 0 sets of 0 points, dt = 1
There are 1 bins in the 0-dimensional histogram. Beta-Emin = 0
Ptot = 0
Back Off! I just backed up ener.xvg to ./#ener.xvg.1#
GROMACS reminds you: “Don’t pay any attention to what they write about you. Just measure it in inches.” (Andy Warhol)
Before this command, I run “gmx covar” and “gmx anaeig” with backbone, caused by the Segmentation fault which means too many atoms in ‘Protein’.For ‘-s md.tpr -f md_center.xtc’ are generated by ‘Protein’.