GROMACS version:2021.4
GROMACS modification: Yes/No
Here post your question : I want to simulate my protein at water-cyclohexane interface. Can I use gmx insert-molecules to insert cyclohexane molecules in a box with protein centered in it, and then add water, instead of adding a pre-equilibrated box of cyclohexane followed by adding water (as given in gromacs tutorial page for biphasic systems). Can anyone please help with this?