Protein-ligand rmsf graph,g_rmsf: command not found

[imrbay]

GROMACS version:2020
GROMACS modification: Yes/No
Here post your question
my system is protein-ligand.ı want to calculate rmsf based on backbone of protein.
my graph is

but when I used - res for backbone rmsf
my graph is

what is the problem this graph,
also,g_rmsf -s step5.tpr -f new.xtc -o yeni_rmsf.xvg -oq rmsf.pdb -res
g_rmsf: command not found not working
Can anybody explain me

[cathrine]

Dear Imrbay,
There’s no command called g_rmsf in GROMACS 2020. Type gmx rmsf -h and you’ll get information about how to compute the RMSF.