Protein ns MD simulation and other times recommended inquiry?

There is no need to equilibrate for 1 microsecond at each interval of equilibration. These steps merely relax restraints to prepare the system. Your production (unrestrained) simulation should be on the microsecond scale. I would suggest following the CHARMM-GUI protocol exactly, as it is very well validated.

Hi Justin thank you for your kind update:)

I will adjust/monitor parameters.