Statistical Analysis Method for Peak force and total work in Steered molecular dynamics

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Is a two-sample t-test reasonable for comparing SMD results between groups?

If not T-test then what other statistical method would be appropriate and why?

I have four groups (protein-protein), 10 replicates for each protein-protein group. I want to plot the graph and perform statistical analysis for peak force and total work across 4 groups.

To create the replicates within each SMD group, I used

  • Same pressure-equilibrated .gro starting structure

  • Different velocity seeds for each replicate

  • Same pulling protocol for all groups

Would you treat the replicates within each group as an independent enough for pairwise t-tests?