Halo Experts!
I am trying to calculate autocorrelation force for each of 24 windows generated after umbrella sampling of drug pull to the centre of lipid bilayer. I am presenting here one command line for window 2.4. Please help me out to write correct command line/s. Should i use normalize or nonormalize? Should I add flags like -subav, -acflen etc?
gmx analyze -f md2.4_pullf.xvg -nonormalize -ac acf2.4.xvg -integrate
or should i write
gmx analyze -f md2.4_pullf.xvg -ac acf2.4.xvg -subav -nonormalize
…please help