DSSP stuck

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1

GROMACS version:2022.3

I am analyzing a trajectory using gmx do_dssp (GROMACS 2022.3) for an intrinsically disordered protein (IDP) simulated in presence of osmolytes.

My issue:
gmx do_dssp consistently freezes when it reaches a specific region of the trajectory (~410–450 ns).
The freeze occurs with both:

  • classic DSSP 2.0.4

  • mkdssp 3.0.0

I have verified that the simulation trajectory is valid:

  • gmx check reports no missing/incomplete/corrupted frames

  • RMSD, Rg, RMSF, and all other analyses run without any issue

  • The xtc file is fully readable

  • Other tools (trjconv, trjcat, gmx rms) process the same time-window normally

However, DSSP always gets stuck at the same point.

Anyone please help me with this.