Perform WHAM with rotation output file from enforced rotation md simulations for umbrella sampling

GROMACS version: 2021
GROMACS modification: Yes/No
Hi everybody,

I performed an umbrella sampling using as CV the rotation angle of protein helix. But I didn’t use pulling md to produce configurations. I used enforced rotation to generate the configurations. And my question is: if can I use WHAM analysis tool with the output files generated from enforced rotation as input files?

Note: To perform umbrella sampling I based on gromacs tutorial of umbrella sampling and performed at each configuration the enforced rotation with 200kj/mol of applied force and 0 degrees/ps of angle rate. So the helix will not rotate at the simulations.