Temperature in pr.mdp file

GROMACS version: 5.1.4
GROMACS modification: Yes/No
Dear all,
We are performing MD simulations of protein at 350 K , we want to know that should I change the ref_t and gen_temp in position restrain pr.mdp file to 350 K or is kept at 300 K.
Anybody can suggest me regarding this.

Hi,
since you want to equilibrate at 350 K both ref_t (barostat reference temperature) and gen_temp (temperature for which you ask to generate velocities) have to be 350 K.
Best regards
Alessandra

Thank you for your response. Since we are doing full MD at 350 K then should we maintain reference temperature of position restrain file at 350 K. kindly suggest me