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I plotted a free energy landscape plot in gromacs using radius of gyration and end to end distance with gmx sham . How to find out the convergence of the FEL plot . Does it depend on convergence of the two reaction coordinates ?

Convergence requires an examination of the change in the quantity(ies) of interest over time. Perform the analysis over consecutive blocks of simulation time and see if the result changes. If it doesnâ€™t (within statistical fluctuation), the result is converged.