Gromacs Mean Square Displacement (gmx_msd) issue

GROMACS version:2024:2
GROMACS modification: Yes/No

I am trying to caculate MSD-center of mass of sukpnated polystyrene.
I have used below to calculate MSD in previous versions successfully for 500 frames .
gmx msd -f pss10_500_1000.xtc -s pss10.gro -n pss10.ndx -o msd-pss10.xvg
But now I get below when I try to run this and it does not giving any out put.Please advice.
“Notofocation I get”:
Available static index groups:
** Group 0 “Group” (274891 atoms)**
Specify any number of selections for option ‘sel’
(Selections to compute MSDs for from the reference):
(one per line, for status/groups, ‘help’ for help, Ctrl-D to end)

Does your index file contain the relevant groups?

It has only one group set
That file appears as below;

[ System ]
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40 41 42 43 44 45
46 47 48 49 50 51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70 71 72 73 74 75
76 77 78 79 80 81 82 83 84 85 86 87 88 89 90
91 92 93 94 95 96 97 98 99 100 101 102 103 104 105
106 107 108 109 110 111 112 113 114 115 116 117 118 119 120
121 122 123 124 125 126 127 128 129 130 131 132 133 134 135
136 137 138 139 140 141 142 143 144 145 146 147 148 149 150
151 152 153 154 155 156 157 158 159 160 161 162 163 164 165
166 167 168 169 170 171 172 173 174 175 176 177 178 179 180
181 182 183 184 185 186 187 188 189 190 191 192 193 194 195
196 197 198 199 200 201 202 203 204 205 206 207 208 209 210
211 212 213 214 215 216 217 218 219 220 221 222 223 224 225
226 227 228 229 230 231 232 233 234 235 236 237 238 239 240
241 242 243 244 245 246 247 248 249 250 251 252 253 254 255
256 257 258 259 260 261 262 263 264 265 266 267 268 269 270
271 272 273 274 275 276 277 278 279 280 281 282 283 284 285
Let me know if you think there is any issue in it

Then it is strange that you get Group 0 “Group” (274891 atoms) to choose from.

Is there any advice that you can give me to try?
I have total 274891 atoms in the index file ,In earlier reply I just included only the intial lines.I have removed around 1000 sodium counter ions and created the index file having only 274891 atoms in polymer chains.In gro file it has the total atoms with sodium 275891.

First, I would recommend making a selection group for the atoms you are interested in calculating the MSD of, i.e. the polystyrene - not the solvent.

It’s a bit unclear to me, where the program stops. You just say it’s “not giving any out put”, but you don’t explicitly say that you have provided the numbers of the selection groups you want to analyze. Have you? If you did so, I think the problem is that it might be slow (and quite irrelevant) calculating the MSD of all 274891 atoms. How long did you wait?