Is there a way to pull ligand into the protein binding site?

GROMACS version: 2018
GROMACS modification: No

I have seen works on pulling of ligand into GPCR using Supervised MD. Is there a way to implement such simulations in Gromacs? Something like a ‘reverse’ pulling ( commonly used in umbrella sampling).

I am not familiar with Supervised MD. But you can perform umbrella sampling using GROMACS. See for details on the

Best regards