Fatal error: Topology include file "gromos53a6_lipid.ff/forcefield.itp" not found; MEMBRANE PROTEIN

GROMACS version: 2022.1
GROMACS modification: No
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Hello and thank you for considering this:):

For the below subsection:

Membrane Protein: KALP15 in DPPC

Step Three: Defining the Unit Cell & Adding Solvent

1. Orient the protein and membrane

I adjusted my forcefield appropriately I believe previously and input the below command:

gmx grompp -f minim.mdp -c dppc128.pdb -p topol_dppc.top -o dppc.tpr

and received the following Fatal Error:
Fatal error:
Topology include file “gromos53a6_lipid.ff/forcefield.itp” not found

The forcefield.itp file is present (see.screenshot attached) accordingly maybe I just need to
adjust its position? And or something else?

Thanks:)
Joel

You put it in the source code but that doesn’t make it available when actually running grompp, unless you also specified that the force field be added to the installation target list. The tutorial tells you to simply create the force field directory in the working directory.

Hi Justin thanks a bunch:)