Topology include file "./amber14sb_OL15.ff/forcefield.itp" not found

GROMACS version: 2020
GROMACS modification: Yes/No
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When I tried to run the follwing command to add the ions to neutralize the system
gmx grompp -f ions.mdp -c protein_solv.gro -p -o ions.tpr

I am always getting this error, I am sure all files are in the same directory

Fatal error:
Topology include file “./amber14sb_OL15.ff/forcefield.itp” not found

do I need to write a special mdp file for my system ?


it says you dont have that particular file in the directory.


the problem is that the force field files are not in the directory defined in the topology file.
At begin of you have defined the path where force field files are using #include"…", check that the path is correct.

My suggestion is to put always the full path for the force field files to avoid problems when the location of changes.

Kind regards

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