Overriding Proper Dih. parameters

GROMACS version: VERSION 5.1.1
GROMACS modification: Yes/No
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Dear experts,
While using ions.tpr command line I am facing errors. Forcefield used: charmm36-mar2019.ff
Command line:
gmx grompp -f ions.mdp -c solv.gro -p topol.top -o ions.tpr

Ignoring obsolete mdp entry ‘title’

Back Off! I just backed up mdout.mdp to ./#mdout.mdp.2#

NOTE 1 [file ions.mdp]:
With Verlet lists the optimal nstlist is >= 10, with GPUs >= 20. Note
that with the Verlet scheme, nstlist has no effect on the accuracy of
your simulation.

Setting the LD random seed to 2813366769

WARNING 1 [file drg.prm, line 4]:
Overriding Bond parameters.

old: 0.1475 234304 0.1475 234304
new: CG2O1 CG2R51 1 0.14750000 251040.00

WARNING 2 [file drg.prm, line 17]:
Overriding U-B parameters.

old: 122 376.56 0 0 122 376.56 0 0
new: CG2R51 CG2O1 OG2D1 5 121.000000 251.040000 0.00000000 0.00

WARNING 3 [file drg.prm, line 19]:
Overriding U-B parameters.

old: 130 292.88 0 0 130 292.88 0 0
new: CG2O1 CG2R51 CG2R51 5 130.000000 383.254400 0.00000000 0.00

WARNING 4 [file drg.prm, line 35]:
Overriding Proper Dih. parameters.
Use dihedraltype 9 to allow several multiplicity terms. Only consecutive
lines are combined. Non-consective lines overwrite each other.

old: 180 7.1128 2 180 7.1128 2
new: OG2D1 CG2O1 CG2R51 CG2R51 9 180.000000 4.184000 2

Generated 100032 of the 100128 non-bonded parameter combinations
Generating 1-4 interactions: fudge = 1
Generated 65937 of the 100128 1-4 parameter combinations
Excluding 3 bonded neighbours molecule type ‘Protein’
Excluding 3 bonded neighbours molecule type ‘DRG’

NOTE 2 [file topol.top, line 61631]:
System has non-zero total charge: 3.000004
Total charge should normally be an integer. See
Floating point arithmetic — GROMACS webpage https://www.gromacs.org documentation
for discussion on how close it should be to an integer.


Program gmx grompp, VERSION 5.1.1
Source code file: /home/cl_dop/Downloads/gromacs/gromacs-5.1.1/src/gromacs/gmxpreprocess/grompp.c, line: 605

Fatal error:
number of coordinates in coordinate file (solv.gro, 55333)
does not match topology (topol.top, 6512)
For more information and tips for troubleshooting, please check the GROMACS
website at Common Errors — GROMACS webpage https://www.gromacs.org documentation

Please help!

The March 2019 port is quite outdated and CGenFF parameters have been updated since then. Use our latest (July 2022) CHARMM36 port if you’re using the CGenFF web server to generate the ligand topology.

Thank you so much sir for your response!