Simulated annealing production run. How can one plot comparative RMSD plot?

GROMACS version: 2021
GROMACS modification: No

Good day,

I am trying to perform simulated annealing of a protein (heating from 300K to 350K).

I performed generic energy minimization and NVT steps, after that I performed NPT with simulated annealing (300 340). For the production run, I used NPT ensemble.

I want to make RMSD plot showing the graphs at 300K and 350K.

Should I make two separate production runs at 300K and 350K ? Or is there a way to extract these data from a single simulated annealing run?

Thank you

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