I am getting this warning when I am performing Energy minimization

User discussions
1

NOTE 3 [file topol.top, line 44]:
System has non-zero total charge: 0.179030
Total charge should normally be an integer. See
Floating point arithmetic — GROMACS webpage https://www.gromacs.org documentation
for discussion on how close it should be to an integer.

WARNING 1 [file topol.top, line 44]:
You are using Ewald electrostatics in a system with net charge. This can
lead to severe artifacts, such as ions moving into regions with low
dielectric, due to the uniform background charge. We suggest to
neutralize your system with counter ions, possibly in combination with a
physiological salt concentration.

2

As the note and warning say, you have a non-integer total charge in the system. That indicates that something is not correctly setup. The first thing you should do is to look through your topology. There must be at least one molecule with non-integer charges.

But without more information about how you generated your topology, it is difficult to help.