What is the cause of membrane rupture?

GROMACS version:2021
GROMACS modification: Yes/No
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I did the ioniization and energy minimization for dppc membrane along with interested nanoparticle, but structure disintegrated and have some anomalies. pbc condition was removed using the following command but the problem still exists.
gmx trjconv -f em.gro -s em.tpr -o nopbc.pdb -ur compact -center -pbc nojump

Please don’t post duplicate topics. I’ll comment in the existing thread where you first raised this issue and you should consider deleting this one, please.